Micropropagation of carnation ( Dianthus caryophyllus L . ) in liquid medium by temporary immersion bioreactor in comparison with solid culture
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چکیده
منابع مشابه
Production of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture
Doubled haploids of carnation (Dianthus caryophyllus L.) were obtained by pseudofertilized ovule culture. Emasculated ̄ower buds of carnation were pollinated with pollen inactivated by Xray irradiation. After 2±3 weeks, the ovaries were explanted and were cultured on solid MS medium containing 2 mM NAA, 2 mM BAP and 6% sucrose. Regenerated plants(R0) were morphologically different from the moth...
متن کاملSequence Analysis of the Genome of Carnation (Dianthus caryophyllus L.)
The whole-genome sequence of carnation (Dianthus caryophyllus L.) cv. 'Francesco' was determined using a combination of different new-generation multiplex sequencing platforms. The total length of the non-redundant sequences was 568,887,315 bp, consisting of 45,088 scaffolds, which covered 91% of the 622 Mb carnation genome estimated by k-mer analysis. The N50 values of contigs and scaffolds we...
متن کاملEffects of liquid, temporary immersion bioreactor and solid culture systems on micropropagation of Lilium ledebourii via bulblet microscales— An endangered valuable plant with ornamental potential
Lilium ledebourii (Baker) Boiss. (Liliaceae) is a critically endangered lily species native tonorthern Iran, where it is protected by law. In order to develop a cost effective method for largescalepropagation, the effects of three culture systems (solid, liquid and temporary immersion)and two types of cytokinins [6-Benzyladenine (BA) and Thidiazuron (TDZ)] were studied onthe in vitro plant rege...
متن کاملIn Vitro Culture of Carnation (Dianthus caryophyllus L.) Focusing on the Problem of Vitrification
This study was conducted to evaluate the effect of plant growth regulators on in vitro shoot multiplication, vitrification and rooting of two carnation cultivars (Eskimo Mogr and Innove Orange Bogr). Isolated axillary buds were cultured on MS medium supplemented with different levels of Benzyl amino purine (BAP) or kinetin (Kin) in combination with 0.2 mg/l NAA and shoot multiplication and vitr...
متن کاملComparison of mRNA levels of three ethylene receptors in senescing flowers of carnation (Dianthus caryophyllus L.).
Three ethylene receptor genes, DC-ERS1, DC-ERS2 and DC-ETR1, were previously identified in carnation (Dianthus caryophyllus L.). Here, the presence of mRNAs for respective genes in flower tissues and their changes during flower senescence are investigated by Northern blot analysis. DC-ERS2 and DC-ETR1 mRNAs were present in considerable amounts in petals, ovaries and styles of the flower at the ...
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ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2017
ISSN: 1687-157X
DOI: 10.1016/j.jgeb.2017.07.005